NIMTE OpenIR  > 2015专题
A novel Trojan-horse targeting strategy to reduce the non-specific uptake of nanocarriers by non-cancerous cells
Shen, Zheyu; Wu, Hao; Yang, Sugeun; Ma, Xuehua; Li, Zihou; Tan, Mingqian; Wu, Aiguo
2015
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume70Pages:1-11
AbstractOne big challenge with active targeting of nanocarriers is non-specific binding between targeting molecules and non-target moieties expressed on non-cancerous cells, which leads to non-specific uptake of nanocarriers by non-cancerous cells. Here, we propose a novel Trojan-horse targeting strategy to hide or expose the targeting molecules of nanocarriers on-demand. The non-specific uptake by non-cancerous cells can be reduced because the targeting molecules are hidden in hydrophilic polymers. The nanocarriers are still actively targetable to cancer cells because the targeting molecules can be exposed on-demand at tumor regions. Typically, Fe3O4 nanocrystals (FN) as magnetic resonance imaging (Mm) contrast agents were encapsulated into albumin nanopartides (AN), and then folic acid (FA) and pH-sensitive polymers (PP) were grafted onto the surface of AN-FN to construct PP-FA-AN-FN nanoparticles. Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), transmission electron microscope (TEM) and gel permeation chromatography (GPC) results confirm successful construction of PP-FA-AN-FN. According to difference of nanoparticle-cellular uptake between pH 7.4 and 5.5, the weight ratio of conjugated PP to nanoparticle FA-AN-FN (i.e. graft density) and the molecular weight of PP (i.e. graft length) are optimized to be 132 and 5.7 kDa, respectively. In vitro studies confirm that the PP can hide ligand FA to prevent it from binding to cells with FR alpha at pH 7.4 and shrink to expose FA at pH 5.5. In vivo studies demonstrate that our Trojan-horse targeting strategy can reduce the nonspecific uptake of the PP-FA-AN-FN by non-cancerous cells. Therefore, our PP-FA-AN-FN might be used as an accurately targeted MRI contrast agent. (C) 2015 Elsevier Ltd. All rights reserved.
Document Type期刊论文
Identifierhttp://ir.nimte.ac.cn/handle/174433/12303
Collection2015专题
Recommended Citation
GB/T 7714
Shen, Zheyu,Wu, Hao,Yang, Sugeun,et al. A novel Trojan-horse targeting strategy to reduce the non-specific uptake of nanocarriers by non-cancerous cells[J]. BIOMATERIALS,2015,70:1-11.
APA Shen, Zheyu.,Wu, Hao.,Yang, Sugeun.,Ma, Xuehua.,Li, Zihou.,...&Wu, Aiguo.(2015).A novel Trojan-horse targeting strategy to reduce the non-specific uptake of nanocarriers by non-cancerous cells.BIOMATERIALS,70,1-11.
MLA Shen, Zheyu,et al."A novel Trojan-horse targeting strategy to reduce the non-specific uptake of nanocarriers by non-cancerous cells".BIOMATERIALS 70(2015):1-11.
Files in This Item:
File Name/Size DocType Version Access License
2015-沈折玉-Biomaterial(2350KB) 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Shen, Zheyu]'s Articles
[Wu, Hao]'s Articles
[Yang, Sugeun]'s Articles
Baidu academic
Similar articles in Baidu academic
[Shen, Zheyu]'s Articles
[Wu, Hao]'s Articles
[Yang, Sugeun]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Shen, Zheyu]'s Articles
[Wu, Hao]'s Articles
[Yang, Sugeun]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.