NIMTE OpenIR  > 2018专题
Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk
Han, Jiaojiao; Zhang, Lei; Hu, Liming; Xing, Keyu; Lu, Xuefei; Huang, Youju; Zhang, Jiawei; Lai, Weihua; Chen, Tao
2018
Source PublicationJOURNAL OF DAIRY SCIENCE
Volume101Issue:7Pages:5770-5779
AbstractLateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA. was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 x 10(2) cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples.
KeywordE. Coli O157h7 Listeria-monocytogenes Nanoparticles Amplification Immunoassay Diagnosis Strip Infections Biosensor O157/h7
Subject AreaMaterials Science ; Physics
Language英语
Document Type期刊论文
Identifierhttp://ir.nimte.ac.cn/handle/174433/17351
Collection2018专题
Recommended Citation
GB/T 7714
Han, Jiaojiao,Zhang, Lei,Hu, Liming,et al. Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk[J]. JOURNAL OF DAIRY SCIENCE,2018,101(7):5770-5779.
APA Han, Jiaojiao.,Zhang, Lei.,Hu, Liming.,Xing, Keyu.,Lu, Xuefei.,...&Chen, Tao.(2018).Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk.JOURNAL OF DAIRY SCIENCE,101(7),5770-5779.
MLA Han, Jiaojiao,et al."Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk".JOURNAL OF DAIRY SCIENCE 101.7(2018):5770-5779.
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